By default, CoinTracking uses an overall method across all exchanges/wallets and calculates gains purely with cost bases by date sequence. By activating depot separation, all exchanges and wallets are treated as separate depots (tax lots). Every different name in the field "exchange" count as separate depot which could also be a wallet name.
This new depot separation automatically recognizes all deposits and withdrawals and assigns them to the respective exchanges/wallets. All previous assignments (from the old depot separation method) are no longer needed or used.
Please ensure that all your deposits and withdrawals between exchanges/wallets are set completely and correctly (withdrawal before deposit!) in your CoinTracking account when enabling this setting.